Article
Neutralization and fusion inhibition activities of monoclonal antibodies specific for the S1 subunit of the spike protein of neurovirulent murine coronavirus JHMV c1-2 variant.
The Journal of general virology - 1 Jul 1993
Kubo H, Takase-Yoden S, Taguchi F
Abstract excerpt
The cleavage products of the spike (S) protein, the S1 and S2 subunits, of the highly neurovirulent murine coronavirus (MHV) JHMV c1-2 variant were identified by immunoprecipitation of virus-infected cell lysates after treatment with urea and 2-mercaptoethanol. By this method 14 monoclonal antibodies (MAbs) raised against the S protein of the c1-2 variant were revealed to react with the S1 subunit and one with...
Topics
- Animals
- Antibodies, Monoclonal
- Antigens, Viral
- Blotting, Western
- Cells, Cultured
- Coronaviridae
- Electrophoresis, Polyacrylamide Gel
- Epitopes
- Genetic Variation
- Macromolecular Substances