Article
Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.
The Journal of general virology - 1 Dec 2000
Taguchi Fumihiro, Shimazaki Yohko K
Abstract excerpt
The monoclonal antibody (MAb) 5B19.2, which has virus-neutralizing and fusion inhibition activities, binds to an epitope (S2A) consisting of nine hydrophobic amino acids in the S2 subunit of the mouse hepatitis virus (MHV) spike (S) protein. This suggests that the S2A epitope may be involved in binding the virus to the MHV receptor and/or in virus-cell fusion. Co-immunoprecipitation analyses demonstrated that...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antigens, Viral
- Cell Fusion
- Cell Line
- Epitopes
- Membrane Glycoproteins
- Molecular Sequence Data
- Murine hepatitis virus
- Mutation
- Neutralization Tests
- Precipitin Tests
- Receptors, Virus
- Spike Glycoprotein, Coronavirus
- Viral Envelope Proteins
- Viral Fusion Proteins