Selection of tRNA(Asp) amber suppressor mutants having alanine, arginine, glutamine, and lysine identity.

Article

Selection of tRNA(Asp) amber suppressor mutants having alanine, arginine, glutamine, and lysine identity.

RNA (New York, N.Y.) - 1 Sept 1996

Martin F, Reinbolt J, Dirheimer G, Gangloff J, Eriani G

Abstract excerpt

Elements that confer identity to a tRNA in the cellular environment, where all aminoacyl-tRNA synthetases are competing for substrates, may be delineated by in vivo experiments using suppressor tRNAs. Here we describe the selection of active Escherichia coli tRNAAsp amber mutants and analyze thei...

Topics

Alanine
Arginine
Base Sequence
Escherichia coli
Genes, Suppressor
Glutamine
Lysine
Models, Genetic
Molecular Sequence Data
Mutation
RNA, Transfer, Asp
Selection, Genetic
Suppression, Genetic

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