Article
Selection of tRNA(Asp) amber suppressor mutants having alanine, arginine, glutamine, and lysine identity.
RNA (New York, N.Y.) - 1 Sept 1996
Martin F, Reinbolt J, Dirheimer G, Gangloff J, Eriani G
Abstract excerpt
Elements that confer identity to a tRNA in the cellular environment, where all aminoacyl-tRNA synthetases are competing for substrates, may be delineated by in vivo experiments using suppressor tRNAs. Here we describe the selection of active Escherichia coli tRNAAsp amber mutants and analyze their identity. Starting from a library containing randomly mutated tRNA(CUA)Asp genes, we isolated four amber suppressors...
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