In vitro selection of small RNAs that bind to Escherichia coli phenylalanyl-tRNA synthetase.

doi:10.1006/jmbi.1994.1571

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Article

In vitro selection of small RNAs that bind to Escherichia coli phenylalanyl-tRNA synthetase.

Journal of molecular biology - 23 Sept 1994

Peterson E T, Pan T, Coleman J, Uhlenbeck O C

Abstract excerpt

Small RNAs were selected from a highly degenerate library on the basis of their ability to bind tightly to Escherichia coli phenylalanyl-tRNA synthetase (FRS). The 63 nucleotide library consisted of the acceptor stem and portions of the D and T stems of E. coli tRNA(Phe) flanking a 32 nucleotide...

Topics

Base Sequence
Cloning, Molecular
Codon
Escherichia coli
Gene Library
Kinetics
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Phenylalanine-tRNA Ligase
Protein Binding
RNA, Transfer, Phe
Sequence Analysis, DNA

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