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Article

A yeast knockout strain to discriminate between active and inactive tRNA molecules.

Nucleic acids research - 15 Aug 2003

Geslain Renaud, Martin Franck, Camasses Alain, Eriani Gilbert

Abstract excerpt

Here we report the construction of a yeast genetic screen designed to identify essential residues in tRNA(Arg). The system consists of a tRNA(Arg) knockout strain and a set of vectors designed to rescue and select for variants of tRNA(Arg). By plasmid shuffling we selected inactive tRNA mutants that were further analyzed by northern blotting. The mutational analysis focused on the tRNA D and anticodon loops that...

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Identifiers

PMID: 12907713
DOI: 10.1093/nar/gkg685
PMCID: PMC169964

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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