Article
Influence of protein surface charge on the bimolecular kinetics of a potassium channel peptide inhibitor.
Biochemistry - 13 Jul 1993
Escobar L, Root M J, MacKinnon R
Abstract excerpt
This study investigates the influence of a through-solution electrostatic interaction on the kinetics of ion channel blockade by the high-affinity peptide inhibitor Lq2. Membrane patches containing many Shaker K+ channels were removed from Xenopus oocytes and placed in a rapid perfusion chamber. Lq2 association and dissociation rate constants were determined from the relaxations to equilibrium blockade following...
Topics
- Animals
- Charybdotoxin
- Electrochemistry
- Kinetics
- Mutation
- Peptides
- Potassium Channels
- Protein Binding
- Scorpion Venoms
- Scorpions
- Xenopus