Article
Use of the single-strand conformation polymorphism technique to detect loss of heterozygosity in neuroblastoma.
Genes, chromosomes & cancer - 1 Jun 1993
White P S, Kaufman B A, Marshall H N, Brodeur G M
Abstract excerpt
Human neuroblastomas are characterized by cytogenetic and molecular analysis as frequently containing deletions of distal 1p. Loss of heterozygosity (LOH) studies have localized a region of shared deletion to 1p35-36.1. Using the single-strand conformation polymorphism (SSCP) technique, we developed polymorphic assays for two genes, the amiloride-sensitive Na+/H+ antiporter (APNH) and tumor necrosis factor...
Topics
- Alleles
- Amiloride
- Base Sequence
- Carrier Proteins
- DNA, Single-Stranded
- Gene Deletion
- Heterozygote
- Humans
- Molecular Sequence Data
- Neuroblastoma
- Polymerase Chain Reaction