Article
Identification of a region within M1 RNA of Escherichia coli RNase P important for the location of the cleavage site on a wild-type tRNA precursor.
Journal of molecular biology - 5 Jun 1993
Kirsebom L A, Svärd S G
Abstract excerpt
To investigate the function of the catalytic subunit of Escherichia coli RNase P, M1 RNA, we studied cleavage by different M1 RNA mutants of wild-type precursors to tRNA(TyrSu3), tRNA(His) and tRNA(SerSu1). We showed that deletion or substitution of the conserved nucleotides G291, G292, U294 and A295 in M1 RNA resulted in a shift of the cleavage site for the tRNA(SerSu1) precursor, whereas the other two tRNA...
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