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Article

Use of RNase P for efficient preparation of yeast tRNATyr transcript and its mutants.

Journal of biochemistry - 1 Jan 2006

Fukunaga Jun-Ichi, Gouda Masaki, Umeda Koji, Ohno Satoshi, Yokogawa Takashi, Nishikawa Kazuya

Abstract excerpt

Because T7 RNA polymerase has a strong preference for particular sequences to initiate transcription, some RNAs having pyrimidine-rich sequences at their 5'-end (yeast tRNA(Tyr), for example) are hardly transcribed by this enzyme. To circumvent this inconvenience, we have developed an efficient method for in vitro preparation of such tRNAs. The RNA of interest is first transcribed as a precursor form that has...

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PMID: 16428327
DOI: 10.1093/jb/mvj005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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