Article
Comprehensive screening of the human KRAS2 gene for sequence variants.
Genes, chromosomes & cancer - 1 Feb 1993
Abrams E S, Murdaugh S E, Lerman L S
Abstract excerpt
To search for mutations in the human KRAS2 oncogene, we have analyzed polymerase chain reaction (PCR) fragments by denaturing gradient gel electrophoresis. We used six different PCR fragments to screen the five coding exons of this gene as well as the splice sites. GC clamps were added to each fr...
Topics
- Base Sequence
- DNA Mutational Analysis
- DNA Probes
- Electrophoresis, Polyacrylamide Gel
- Exons
- Genes, ras
- Genetic Variation
- Humans
- Molecular Sequence Data
- Nucleic Acid Denaturation
- Polymerase Chain Reaction
- Software