Article
Detection of N-, H-, and KRAS codons 12, 13, and 61 mutations with universal RAS primer multiplex PCR and N-, H-, and KRAS-specific primer extension.
Clinical biochemistry - 1 Feb 2010
Chang Ya-Sian, Yeh Kun-Tu, Hsu Nicholas C, Lin Shu-Hui, Chang Tien-Jye, Chang Jan-Gowth
Abstract excerpt
OBJECTIVES: Mutations of all three RAS genes, N-, H-, and KRAS, are identified mainly in codons 12, 13, and 61 of exons 2 and 3 in human cancers. DESIGN AND METHODS: DNA samples were isolated from 58 oral cancer and 106 colorectal cancer patients. Multiplex amplification of codons 12 and 13 of exon 2 and codon 61 of exon 3 of three RAS genes using two pairs of universal primers for exons 2 and 3 was performed in...
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