Article
Identification of regions in polyomavirus middle T and small t antigens important for association with protein phosphatase 2A.
Journal of virology - 1 Jun 1995
Campbell K S, Auger K R, Hemmings B A, Roberts T M, Pallas D C
Abstract excerpt
Two subunits of protein phosphatase 2A (PP2A) have been shown previously to bind to the small t and middle T antigens (ST and MT, respectively) of polyomavirus. To determine sequences important for binding of PP2A to ST and MT, we first constructed a series of ST mutants in regions known to be important for biological activity of ST and MT. Several mutations in two small regions just amino terminal to the...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Antigens, Polyomavirus Transforming
- Base Sequence
- Cell Line, Transformed
- DNA Primers
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mutation