Article
One-step magnetic purification of recombinant DNA-binding proteins using magnetizable phosphocellulose.
Protein expression and purification - 1 Jun 1995
Risøen P A, Struksnes K, Myrset A H, Gabrielsen O S
Abstract excerpt
Magnetizable solid phase technology was used to develop a method for the rapid purification of recombinant proteins expressed in Escherichia coli. We describe the purification of two recombinant DNA-binding proteins: the minimal DNA-binding domain of the oncoprotein Myb and full-length yeast TFIIIA. Both were purified in one step directly from an E. coli lysate by means of magnetizable phosphocellulose particles...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cellulose
- Chickens
- DNA-Binding Proteins
- Escherichia coli
- Magnetics
- Molecular Sequence Data
- Mutation
- Oncogene Proteins v-myb