Article
Highly resolving two-dimensional gels for protein sequencing.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1991
Hanash S M, Strahler J R, Neel J V, Hailat N, Melhem R, Keim D, Zhu X X, Wagner D, Gage D A, Watson J T
Abstract excerpt
Two-dimensional (2D) PAGE, using carrier ampholytes for the first-dimension separation, has provided a tool for the simultaneous analysis of cellular proteins. To extend the utility of 2D PAGE to the preparative level, we have investigated the use of immobilized pH gradients (IPG) for the first-d...
Topics
- Amino Acid Sequence
- Electrophoresis, Gel, Two-Dimensional
- In Vitro Techniques
- Isoelectric Point
- Lymphocytes
- Mass Spectrometry
- Molecular Weight
- Mutation
- Proteins