Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture.

Biotechnology progress - 1 Jan 2000

Yeung Yik A, Wittrup K Dane

Abstract excerpt

Magnetic bead capture is demonstrated here to be a feasible alternative for quantitative screening of favorable mutants from a cell-displayed polypeptide library. Flow cytometric sorting with fluorescent probes has been employed previously for high throughput screening for either novel binders or improved mutants. However, many laboratories do not have ready access to this technology as a result of the limited...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antibodies
Share to Antibody Affinity
Share to Feasibility Studies

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Ligands

Share to Magnetics

Share to Microspheres

Share to Peptide Library

Share to Streptavidin

Source

Identifiers

PMID: 11934287
DOI: 10.1021/bp010186l

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture.PMID 11934287Read ↗

Fine affinity discrimination by yeast surface display and flow cytometry.Semantic proximity Read ↗

Magnetic sorting of membrane associated IgG for phenotype-based selection of stable antibody producing cells.Semantic proximity Read ↗

In vitro affinity screening of protein and peptide binders by megavalent bead surface display.Semantic proximity Read ↗

Multiplex bead binding assays using off-the-shelf components and common flow cytometers.Semantic proximity Read ↗

Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments.Semantic proximity Read ↗

Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).Semantic proximity Read ↗

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function.Semantic proximity Read ↗

Development of a high-throughput label-free method for enrichment of antimetabolite-resistant yeast strains using pico-litre agarose beads.Semantic proximity Read ↗

Development of a novel strategy for engineering high-affinity proteins by yeast display.Semantic proximity Read ↗

Antibody-Free Magnetic Cell Sorting of Genetically Modified Primary Human CD4+ T Cells by One-Step Streptavidin Affinity PurificationSemantic proximity Read ↗

An improved yeast transformation method for the generation of very large human antibody librariesSemantic proximity Read ↗

Evaluation and Selection of Potent Fluorescent Immunosensors by Combining Fluorescent Peptide and Nanobodies Displayed on Yeast SurfaceSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.