Article
Aberrant cGMP-binding activity in non-chemotactic Dictyostelium discoideum mutants.
Biochimica et biophysica acta - 31 Aug 1995
Kuwayama H, Viel G T, Ishida S, Van Haastert P J
Abstract excerpt
The kinetics of cGMP-binding to the major cGMP-binding activity in Dictyostelium were investigated in 10 non-chemotactic mutants (KI mutants; KI-1 approximately 10). A wild-type cell contains about 3000 binding sites with a Kd of 1.5 nM. cGMP may dissociate from these binding sites with fast (F-type) or slow (S-type) kinetics, and DNA has been shown to promote the conversion of F- to S-type of cGMP-binding. The...
Topics
- Animals
- Binding Sites
- Cyclic GMP
- Dictyostelium
- Kinetics
- Mutation