Article
Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene.
Cancer letters - 16 Aug 1995
Ho Y S, Lin J K
Abstract excerpt
DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. H...
Topics
- 2-Acetylaminofluorene
- Acetoxyacetylaminofluorene
- Base Sequence
- DNA Adducts
- DNA Primers
- Dose-Response Relationship, Drug
- Exons
- Genes, p53
- Humans
- In Vitro Techniques
- Molecular Sequence Data
- Mutation
- Nitroso Compounds
- Plasmids