Article
Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides.
Molecular & general genetics : MGG - 1 Feb 1991
Arnau J, Jepsen L P, Strøman P
Abstract excerpt
Transformation of a Mucor circinelloides Leu- strain with the plasmid pAD45, harbouring the wild-type allele (leuA+) and a chymosin gene, led to the identification of mitotically stable transformants after one to three vegetative growth cycles on non-selective medium. Southern analysis of the stable transformed strains demonstrated that the vector is integrated, as an intact molecule, into the resident Mucor leuA...
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