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Article

Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides.

Molecular & general genetics : MGG - 1 Feb 1991

Arnau J, Jepsen L P, Strøman P

Abstract excerpt

Transformation of a Mucor circinelloides Leu- strain with the plasmid pAD45, harbouring the wild-type allele (leuA+) and a chymosin gene, led to the identification of mitotically stable transformants after one to three vegetative growth cycles on non-selective medium. Southern analysis of the stable transformed strains demonstrated that the vector is integrated, as an intact molecule, into the resident Mucor leuA...

Topics

Alleles
Blotting, Southern
Chymosin
Cloning, Molecular
Escherichia coli
Genes, Fungal
Isomerases
Mitosis
Mucor
Plasmids
Recombination, Genetic
Transformation, Genetic

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Identifiers

PMID: 2005861
DOI: 10.1007/BF00269847

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2005861-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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