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Article

Efficient transformation of Claviceps purpurea using pyrimidine auxotrophic mutants: cloning of the OMP decarboxylase gene.

Molecular & general genetics : MGG - 1 Aug 1992

Smit R, Tudzynski P

Abstract excerpt

A homologous transformation system was developed for the phytopathogenic fungus Claviceps purpurea. Orotidine-5'-monophosphate decarboxylase (OMPD)-deficient mutants were obtained by UV mutagenesis and selection for resistance against 5-fluoroorotate. These mutants could be complemented well by the corresponding genes of Aspergillus niger (pyrA) and Neurospora crassa (pyr4), yielding significantly higher...

Topics

Blotting, Southern
Claviceps
Cloning, Molecular
Genomic Library
Mutation
Orotidine-5'-Phosphate Decarboxylase
Plasmids
Pyrimidines
Recombinant Fusion Proteins
Sequence Homology, Nucleic Acid
Transformation, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 1508154
DOI: 10.1007/BF00283850

Genes

OMP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1508154-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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