Article
Analysis of the NH2-terminal 83rd amino acid of Escherichia coli GyrA in quinolone-resistance.
Microbiology and immunology - 1 Jan 1995
Yonezawa M, Takahata M, Banzawa N, Matsubara N, Watanabe Y, Narita H
Abstract excerpt
Artificial mutations of Gyrase A protein (GyrA) in Escherichia coli by site-directed mutagenesis were generated to analyze quinolone-resistant mechanisms. By genetic analysis of gyrA genes in a gyrA temperature sensitive (Ts) background, exchange of Ser at the NH2-terminal 83rd position of GyrA to Trp, Leu, Phe, Tyr, Ala, Val, and Ile caused bacterial resistance to the quinolones, while exchange to Gly, Asn, Lys,...
Topics
- 4-Quinolones
- Anti-Infective Agents
- Base Sequence
- DNA Gyrase
- DNA Primers
- DNA Topoisomerases, Type II
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Site-Directed