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Article

Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein.

Protein science : a publication of the Protein Society - 1 May 2000

Blance S J, Williams N L, Preston Z A, Bishara J, Smyth M S, Maxwell A

Abstract excerpt

Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon...

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Identifiers

PMID: 10850814
DOI: 10.1110/ps.9.5.1035
PMCID: PMC2144630

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Source receipt: pubmed, retrieved 7 Sept 2026.

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