Article
Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein.
Protein science : a publication of the Protein Society - 1 May 2000
Blance S J, Williams N L, Preston Z A, Bishara J, Smyth M S, Maxwell A
Abstract excerpt
Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon...
Topics
- Antibiotics, Antineoplastic
- Coumarins
- Crystallography, X-Ray
- DNA Gyrase
- DNA Topoisomerases, Type II
- Drug Resistance, Neoplasm
- Enzyme Inhibitors
- Escherichia coli
- Glycine
- Models, Chemical
- Models, Molecular
- Mutagenesis, Site-Directed
- Novobiocin