Article
Escherichia coli DNA gyrase: genetic analysis of gyrA and gyrB mutations responsible for thermosensitive enzyme activity.
FEBS letters - 2 Nov 1992
Oram M, Kuroda R, Fisher L M
Abstract excerpt
Escherichia coli gyrA43 and gyrB203 alleles conferring temperature-sensitive (ts) growth encoded Gly751-->Asp and Pro171-->Ser substitutions in the DNA gyrase A and B subunits, respectively. A plasmid-borne gyrA43 allele was genetically dominant over a chromosomal quinolone-resistant gyrA gene at 30 degrees C but not at 42 degrees C. These results and others confirm the ts phenotype of the mutation, the first to...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- DNA Topoisomerases, Type II
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides