Article
Visualization of protein transport along the secretory pathway using green fluorescent protein.
FEBS letters - 7 Aug 1995
Kaether C, Gerdes H H
Abstract excerpt
We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was observed when biosynthetic protein transport was arrested at the intermediate compartment or the...
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