Visualization of protein transport along the secretory pathway using green fluorescent protein.

doi:10.1016/0014-5793(95)00765-2

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Article

Visualization of protein transport along the secretory pathway using green fluorescent protein.

FEBS letters - 7 Aug 1995

Kaether C, Gerdes H H

Abstract excerpt

We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, f...

Topics

Amino Acid Sequence
Base Sequence
Biological Transport
Chromogranins
Cytoplasm
Endoplasmic Reticulum
Fluorescence
Golgi Apparatus
Green Fluorescent Proteins
HeLa Cells
Humans
Luminescent Proteins
Molecular Sequence Data
Mutation
Recombinant Fusion Proteins
Temperature

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