Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice.

Abstract excerpt

The lacZ transgenic mouse (Muta mouse) model was used to examine the timing of ethylnitrosourea (ENU)-induced mutations in germ cells. The spectrum of mutations was also determined. Animals received five daily treatments with ENU at 50 mg/kg and were sampled at times up to 55 days after treatment. In mixed germ-cell populations isolated from seminiferous tubules, there was little increase in the mutant frequency...

Topics

• Animals
• Base Composition
• Base Sequence
• DNA
• Ethylnitrosourea
• Lac Operon
• Male
• Mice
• Mice, Transgenic
• Molecular Sequence Data
• Mutation