Article
Catalytically critical nucleotide in domain 5 of a group II intron.
Proceedings of the National Academy of Sciences of the United States of America - 9 May 1995
Peebles C L, Zhang M, Perlman P S, Franzen J S
Abstract excerpt
Domain 5 (D5) is a small hairpin structure within group II introns. A bimolecular assay system depends on binding by D5 to an intron substrate for self-splicing activity. In this study, mutations in D5 identify two among six nearly invariant nucleotides as being critical for 5' splice junction hy...
Topics
- Base Composition
- Base Sequence
- Binding, Competitive
- Catalysis
- Conserved Sequence
- DNA-Directed RNA Polymerases
- Genetic Variation
- Introns
- Kinetics
- Molecular Sequence Data
- Nucleic Acid Conformation
- Promoter Regions, Genetic
- RNA
- RNA Precursors
- RNA Splicing
- Regression Analysis
- Templates, Genetic
- Thermodynamics