Article
The GTPase activity of the Escherichia coli Ffh protein is important for normal growth.
Biochimica et biophysica acta - 20 Jun 1995
Samuelsson T, Olsson M, Wikström P M, Johansson B R
Abstract excerpt
The Escherichia coli (E. coli) Ffh protein is homologous to the 54kDa subunit of the eukaryotic signal recognition particle. We have examined an intrinsic GTPase activity of this protein and have created mutations in one sequence motif (GXXXXGK) of the putative GTP binding site. When glycine-112 was changed to valine (Ffh-G112V), Vmax was reduced to only 4% of the wildtype level. On the other hand, when...
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