Article
Deletion of the putative effector region of Era, an essential GTP-binding protein in Escherichia coli, causes a dominant-negative phenotype.
FEMS microbiology letters - 15 Sept 1996
Pillutla R C, Ahnn J, Inouye M
Abstract excerpt
Era is an essential gene in E. coli, encoding a GTP-binding protein of unknown function. In the present work, a mutant designated Era-dE, for deletion of effector region is described. This is the first and only known era allele that confers a dominant-negative phenotype. Phenotypic analysis of th...
Topics
- Adenosine Triphosphate
- Bacterial Proteins
- Carbon
- Citric Acid Cycle
- Electrophoresis, Gel, Two-Dimensional
- Escherichia coli
- Escherichia coli Proteins
- GTP Phosphohydrolases
- GTP-Binding Proteins
- Gene Deletion
- Genes, Bacterial
- Glucose
- Mutation
- Phenotype
- RNA-Binding Proteins