Article
Analysis of the GTPase activity and active sites of the NG domains of FtsY and Ffh from Streptomyces coelicolor.
Acta biochimica et biophysica Sinica - 1 Jul 2006
Dong Hui-Jun, Tao Sheng-Mao, Li Yong-Quan, Chan Siu-Hong, Shen Xue-Ling, Wang Chun-Xia, Guan Wen-Jun
Abstract excerpt
Fifty-four homolog (Ffh) and FtsY are the central components of the signal recognition particle secretory pathway of bacteria. In this study, the core domain and active sites of FtsY and Ffh from Streptomyces coelicolor, which are responsible for guanosine triphosphate (GTP) hydrolysis, were identified using site-directed mutagenesis. Mutations were introduced to the conserved GXXGXGK loop of the putative GTP...
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