Article
Mutation analysis in two newly identified rat p53 pseudogenes.
Mutagenesis - 1 Mar 1995
Ciotta C, Dogliotti E, Bignami M
Abstract excerpt
We have analysed the genomic organization of the rat p53 gene in normal intestinal rat cells. Exons 5-8 of the p53 gene were amplified by PCR from rat genomic DNA using primers complementary to stretches of nucleotides identical in rat and human cDNAs. Two amplification products of 727 and 339 bp were obtained from the rat DNA. The PCR products were subcloned into M13mp18 and sequenced. By comparison with the rat...
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