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Article

Polymerization of recombinant Hb S-Kempsey (deoxy-R state) and Hb S-Kansas (oxy-T state).

The Journal of biological chemistry - 10 Nov 1995

Adachi K, Sabnekar P, Adachi M, Reddy L R, Pang J, Reddy K S, Surrey S

Abstract excerpt

In order to investigate the role of the R (relaxed) to T (tense) structural transition in facilitating polymerization of deoxy-Hb S, we have engineered and expressed two Hb S variants which destabilize either T state (Hb S-Kempsey, alpha 2 beta 2 Val-6,Asn-99) or R state structures (Hb S-Kansas, alpha 2 beta 2 Val-6, Thr-102). Polymerization of deoxy-Hb S-Kempsey, which shows high oxygen affinity and increased...

Topics

Carboxyhemoglobin
Genetic Variation
Hemoglobins
Hemoglobins, Abnormal
Humans
Kinetics
Molecular Structure
Oxidation-Reduction
Oxyhemoglobins
Polymers
Protein Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombinant Proteins

Saccharomyces cerevisiae

Source

Identifiers

PMID: 7592928
DOI: 10.1074/jbc.270.45.26857

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7592928-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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