Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP.

doi:10.1093/nar/20.4.871

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Article

Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP.

Nucleic acids research - 25 Feb 1992

Sarkar G, Yoon H S, Sommer S S

Abstract excerpt

Single-strand conformation polymorphism (SSCP) is a simple method for detecting the presence of mutations in a segment of DNA, but the fraction of all mutations detected is unclear. We have evaluated SSCP for the detection of single-base mutations in the factor IX gene. Multiple conditions were e...

Topics

Base Sequence
DNA, Single-Stranded
Electrophoresis
Factor IX
Genetic Testing
Hemophilia B
Humans
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Polymorphism, Genetic
RNA
Temperature

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