Article
Bipolar clamping improves the sensitivity of mutation detection by temperature gradient gel electrophoresis.
Electrophoresis - 1 Jun 1998
Gille C, Gille A, Booms P, Robinson P N, Nürnberg P
Abstract excerpt
Temperature gradient gel electrophoresis (TGGE) is a rapid and sensitive screening method for point mutations and other small DNA alterations. Usually a polymerase chain reaction (PCR)-product of 150 to 500 bp that has been clamped at one end by a psoralen molecule or a "GC-clamp" is tested for abnormal melting characteristics by electrophoresis in a temperature gradient. Under optimal conditions, a heterozygous...
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