A general strategy for producing conditional alleles of Src-like tyrosine kinases.

doi:10.1073/pnas.92.21.9805

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Article

A general strategy for producing conditional alleles of Src-like tyrosine kinases.

Proceedings of the National Academy of Sciences of the United States of America - 10 Oct 1995

Spencer D M, Graef I, Austin D J, Schreiber S L, Crabtree G R

Abstract excerpt

The Src-like tyrosine kinases require membrane localization for transformation and probably for their normal role in signal transduction. We utilized this characteristic to prepare Src-like tyrosine kinases that can be readily activated with the rationally designed chemical inducer of dimerization FK1012. Dimerization of cytoplasmic Src-like tyrosine kinases was not sufficient for signaling, but their recruitment...

Topics

Alkaline Phosphatase
Alleles
Animals
Calcium
Cell Compartmentation
Cells, Cultured
Electroporation
Enzyme Activation
Humans
Lymphocyte Activation
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis

Protein Kinase C

Proto-Oncogene Proteins

Proto-Oncogene Proteins c-fyn

Receptors, Antigen, T-Cell

Recombinant Fusion Proteins

Signal Transduction

Source

Open in PubMed Open full text

Identifiers

PMID: 7568222
DOI: 10.1073/pnas.92.21.9805
PMCID: PMC40891

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7568222-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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