Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.

Journal of bacteriology - 1 Oct 1995

Ponce E, Flores N, Martinez A, Valle F, Bolívar F

Abstract excerpt

We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism. These genes were insertionally inactivated with antibiotic resistance markers and utilized to interr...

Topics

Cloning, Molecular
Escherichia coli
Genes, Bacterial
Gluconates
Glucose
Isoenzymes
Mutation
Pyruvate Kinase
Pyruvates
Pyruvic Acid
Ribose
Sequence Analysis, DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Open in PubMed Open full text

Identifiers

PMID: 7559366
DOI: 10.1128/jb.177.19.5719-5722.1995
PMCID: PMC177388

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7559366-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.PMID 7559366Read ↗

Two isoforms of pyruvate kinase enzymes in Pseudomonas aeruginosa with distinct functional and structural propertiesSemantic proximity Read ↗

Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli.Semantic proximity Read ↗

Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants.Semantic proximity Read ↗

Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli.Semantic proximity Read ↗

Phosphoenolpyruvate synthase plays an essential role for glycolysis in the modified Embden-Meyerhof pathway in Thermococcus kodakarensis.Semantic proximity Read ↗

Conversion of Escherichia coli pyruvate oxidase to an 'alpha-ketobutyrate oxidase'.Semantic proximity Read ↗

Characterization of pyruvate uptake in Escherichia coli K-12.Semantic proximity Read ↗

A polyphosphate kinase (PPK2) widely conserved in bacteriaSemantic proximity Read ↗

High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain.Semantic proximity Read ↗

Pyruvate oxidase contributes to the aerobic growth efficiency of Escherichia coli.Semantic proximity Read ↗

Overproduction and characterization of recombinant UDP-glucose pyrophosphorylase from Escherichia coli K-12.Semantic proximity Read ↗

Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.