Conversion of Escherichia coli pyruvate oxidase to an 'alpha-ketobutyrate oxidase'.

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Article

Conversion of Escherichia coli pyruvate oxidase to an 'alpha-ketobutyrate oxidase'.

The Biochemical journal - 15 Dec 2000

Chang Y Y, Cronan J E

Abstract excerpt

Escherichia coli pyruvate oxidase (PoxB), a lipid-activated homotetrameric enzyme, is active on both pyruvate and 2-oxobutanoate ('alpha-ketobutyrate'), although pyruvate is the favoured substrate. By localized random mutagenesis of residues chosen on the basis of a modelled active site, we obtained several PoxB enzymes that had a markedly decreased activity with the natural substrate, pyruvate, but retained full...

Topics

Amino Acid Substitution
Butyrates
Enzyme Activation
Escherichia coli
Flavin-Adenine Dinucleotide
Ketone Oxidoreductases
Kinetics
Mutation
Pyruvate Oxidase
Pyruvic Acid
Substrate Specificity
Valine

Source

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Identifiers

PMID: 11104678
PMCID: PMC1221509

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