Article
Overproduction and characterization of recombinant UDP-glucose pyrophosphorylase from Escherichia coli K-12.
Journal of biochemistry - 1 May 1994
Hossain S A, Tanizawa K, Kazuta Y, Fukui T
Abstract excerpt
Using oligonucleotide probes synthesized on the basis of partial amino acid sequences, we have cloned and sequenced the gene of Escherichia coli K-12 encoding UDP-glucose pyrophosphorylase. The gene consists of 906 base pairs and encodes a polypeptide of 302 amino acid residues with a calculated...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Sequence Homology, Amino Acid
- UTP-Glucose-1-Phosphate Uridylyltransferase