Article
Solvation energetics and conformational change in EF-hand proteins.
Protein science : a publication of the Protein Society - 1 Feb 2001
Ababou A, Desjarlais J R
Abstract excerpt
Calmodulin and other members of the EF-hand protein family are known to undergo major changes in conformation upon binding Ca(2+). However, some EF-hand proteins, such as calbindin D9k, bind Ca(2+) without a significant change in conformation. Here, we show the importance of a precise balance of solvation energetics to conformational change, using mutational analysis of partially buried polar groups in the...
Topics
- Amino Acid Sequence
- Calbindins
- Calcium
- Calmodulin
- Circular Dichroism
- DNA Mutational Analysis
- Humans
- Kinetics
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Binding
- Protein Conformation
- Protein Structure, Tertiary
- Recombinant Proteins
- S100 Calcium Binding Protein G
- Sequence Homology, Amino Acid