Article
Characterization of the N-terminal part of the neutralizing antigenic site I of coxsackievirus B4 by mutation analysis of antigen chimeras.
Virus research - 1 Nov 1994
McPhee F, Zell R, Reimann B Y, Hofschneider P H, Kandolf R
Abstract excerpt
Coxsackievirus B3 (CVB3) as a potential RNA virus vector for the presentation of foreign antigenic epitopes was further characterized. Insertion mutagenesis of infectious CVB3 cDNA yielded viable antigen chimeras containing variant BC loops of VP1 of coxsackievirus B4 (CVB4). Analysis of three an...
Topics
- Amino Acid Sequence
- Antigens, Viral
- Base Sequence
- DNA Primers
- Enterovirus B, Human
- Epitopes
- Genetic Variation
- Genome, Viral
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed
- Neutralization Tests
- Recombinant Fusion Proteins
- Recombinant Proteins
- Sequence Deletion
- Transfection