Article
Human leukemia K562 cell mutant (K562/OA200) selected for resistance to okadaic acid (protein phosphatase inhibitor) lacks protein kinase C-epsilon, exhibits multidrug resistance phenotype, and expresses drug pump P-glycoprotein.
The Journal of biological chemistry - 22 Apr 1994
Zheng B, Chambers T C, Raynor R L, Markham P N, Gebel H M, Vogler W R, Kuo J F
Abstract excerpt
A human leukemia K562 cell mutant (K562/OA200) selected for resistance to okadaic acid (OA), an inhibitor of protein phosphatases 1 and 2A (PP1/PP2A), has been established. In wild type cells, the cytotoxicity of OA was associated with mitotic arrest and concentration- and time-dependent DNA fragmentation, a hallmark of apoptosis. The mutant was 100-fold more resistant to OA in terms of effects on these...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Antineoplastic Agents
- Blotting, Western
- Carcinogens
- Carrier Proteins
- Cell Division
- Clone Cells
- Cobra Cardiotoxin Proteins
- Dose-Response Relationship, Drug