Mutation detection by mismatch binding protein, MutS, in amplified DNA: application to the cystic fibrosis gene.

doi:10.1073/pnas.91.7.2674

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Article

Mutation detection by mismatch binding protein, MutS, in amplified DNA: application to the cystic fibrosis gene.

Proceedings of the National Academy of Sciences of the United States of America - 29 Mar 1994

Lishanski A, Ostrander E A, Rine J

Abstract excerpt

An experimental strategy for detecting heterozygosity in genomic DNA has been developed based on preferential binding of Escherichia coli MutS protein to DNA molecules containing mismatched bases. The binding was detected by a gel mobility-shift assay. This approach was tested by using as a model...

Topics

Adenosine Triphosphatases
Artifacts
Bacterial Proteins
Base Sequence
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
DNA-Binding Proteins
Escherichia coli Proteins
Frameshift Mutation
Heterozygote
Humans
Membrane Proteins
Molecular Biology
Molecular Sequence Data

Source

Open in PubMed Open full text

Identifiers

PMID: 7511817
DOI: 10.1073/pnas.91.7.2674
PMCID: PMC43432

Genes

CFTR
GAA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7511817-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

MutS DNA Mismatch-Binding Protein

Mutation

Point Mutation

Polymerase Chain Reaction

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.