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Article

Control of ColE2 DNA replication: in vitro binding of the antisense RNA to the Rep mRNA.

Nucleic acids research - 25 Dec 1993

Sugiyama T, Itoh T

Abstract excerpt

Expression of the Rep protein of colicin E2 plasmid is negatively controlled at a post-transcriptional step by a plasmid-coded RNA (RNAI). RNAI is complementary to the 5' nontranslated region of the Rep mRNA and can be folded into two stem-loop structures. Several cop mutations that increased the copy number of the plasmid have been mapped in the RNAI coding region. Here we demonstrated that RNAI and the Rep mRNA...

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Identifiers

PMID: 7507236
DOI: 10.1093/nar/21.25.5972
PMCID: PMC310483

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Research trail→

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Control of ColE2 DNA replication: in vitro binding of the antisense RNA to the Rep mRNA.PMID 7507236Read ↗

Control of ColE2 plasmid replication: regulation of Rep expression by a plasmid-coded antisense RNA.Semantic proximity Read ↗

The effects of RNA degradation enzymes on antisense RNAI controlling ColE2 plasmid copy number.Semantic proximity Read ↗

Control of replication of plasmid R1: the intergenic region between copA and repA modulates the level of expression of repA.Semantic proximity Read ↗

Replication initiator protein mRNA of ColE2 plasmid and its antisense regulator RNA are under the control of different degradation pathways.Semantic proximity Read ↗

Regulation of replication of plasmid R1: an analysis of the intergenic region between copA and repA.Semantic proximity Read ↗

In vivo and in vitro studies of a copy number mutation of the RepA replication protein of plasmid pSC101.Semantic proximity Read ↗

Control of replication of plasmid R1: the duplex between the antisense RNA, CopA, and its target, CopT, is processed specifically in vivo and in vitro by RNase III.Semantic proximity Read ↗

Mutations in an antisense RNA, involved in the replication control of a repABC plasmid, that disrupt plasmid incompatibility and mediate plasmid speciation.Semantic proximity Read ↗

Importance of the leader region of mRNA for translation initiation of ColE2 Rep protein.Semantic proximity Read ↗

Progression of a loop-loop complex to a four-way junction is crucial for the activity of a regulatory antisense RNA.Semantic proximity Read ↗

Positive and negative regulations of plasmid CoLIb-P9 repZ gene expression at the translational level.Semantic proximity Read ↗

Regulation of plasmid R1 replication: PcnB and RNase E expedite the decay of the antisense RNA, CopA.Semantic proximity Read ↗

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