Article
Importance of the leader region of mRNA for translation initiation of ColE2 Rep protein.
Plasmid - 1 Nov 2007
Nagase Tomomi, Nishio Shin-ya, Itoh Tateo
Abstract excerpt
Translation initiation of mRNA encoding the Rep protein of the ColE2 plasmid required for initiation of plasmid DNA replication is fairly efficient in Escherichia coli cells despite the absence of a canonical Shine-Dalgarno sequence. To define sequences and structural elements responsible for translation efficiency of the Rep mRNA, a series of rep-lacZalpha translational fusions bearing various mutations in the...
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