Article
Effects of zinc finger mutations on the nucleic acid binding activities of Xenopus transcription factor IIIA.
Biochemistry - 28 Nov 1995
Zang W Q, Veldhoen N, Romaniuk P J
Abstract excerpt
Transcription factor IIIA (TFIIIA) is required for the activation of 5S RNA gene transcription as well as the storage of 5s RNA as a 7S ribonucleoprotein particle. Interaction with both nucleic acids is mediated through nine C2H2 zinc fingers. In order to determine amino acid regions necessary for nucleic acid interaction, a series of substitution mutants Xenopus laevis TFIIIA have been constructed and expressed...
Topics
- Amino Acid Sequence
- Animals
- DNA-Binding Proteins
- Escherichia coli
- Molecular Sequence Data
- Mutation
- RNA, Ribosomal, 5S
- Recombinant Proteins
- Sequence Alignment
- Transcription Factor TFIIIA
- Transcription Factors