Article
Maximization of selenocysteine tRNA and U6 small nuclear RNA transcriptional activation achieved by flexible utilization of a Staf zinc finger.
The Journal of biological chemistry - 27 Aug 1999
Schaub M, Myslinski E, Krol A, Carbon P
Abstract excerpt
Transcriptional activators Staf and Oct-1 play critical roles in the activation of small nuclear RNA (snRNA) and snRNA-type gene transcription. Recently, we established that Staf binding to the human U6 snRNA (hU6) and Xenopus selenocysteine tRNA (xtRNA(Sec)) genes requires different sets of the seven C2-H2 zinc fingers. In this work, using a combination of oocyte microinjection, electrophoretic mobility shift...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- DNA
- DNA-Binding Proteins
- Host Cell Factor C1
- Humans
- Hydroxyl Radical
- Microinjections
- Molecular Sequence Data
- Mutation
- Octamer Transcription Factor-1
- Oocytes
- Peptide Fragments