Article
Reaction mechanism of chitosanase from Streptomyces sp. N174.
The Biochemical journal - 15 Oct 1995
Fukamizo T, Honda Y, Goto S, Boucher I, Brzezinski R
Abstract excerpt
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of the products were analysed by NMR spectroscopy. The chitosanase produced heterooligosaccharides consisting of D-GlcN and GlcNAc in...
Topics
- Acetylation
- Acetylglucosamine
- Carbohydrate Sequence
- Chitin
- Chitosan
- Chromatography, Gel
- Chromatography, High Pressure Liquid
- Glucosamine
- Glycoside Hydrolases
- Hydrolysis
- Magnetic Resonance Spectroscopy
