Article
A highly Conserved Aspartic Acid Residue of the Chitosanase from Bacillus Sp. TS Is Involved in the Substrate Binding.
Applied biochemistry and biotechnology - 1 Nov 2016
Zhou Zhanping, Zhao Shuangzhi, Liu Yang, Chang Zhengying, Ma Yanhe, Li Jian, Song Jiangning
Abstract excerpt
The chitosanase from Bacillus sp. TS (CsnTS) is an enzyme belonging to the glycoside hydrolase family 8. The sequence of CsnTS shares 98 % identity with the chitosanase from Bacillus sp. K17. Crystallography analysis and site-direct mutagenesis of the chitosanase from Bacillus sp. K17 identified the important residues involved in the catalytic interaction and substrate binding. However, despite progress in...
Topics
- Amino Acid Sequence
- Aspartic Acid
- Bacillus
- Conserved Sequence
- Glycoside Hydrolases
- Kinetics
- Mutant Proteins
- Mutation
- Protein Conformation
- Protein Unfolding
- Sequence Alignment
- Structure-Activity Relationship
- Substrate Specificity
- Temperature