Article
The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a streptomyces enzyme.
Microbiology (Reading, England) - 1 Nov 2000
Rivas Luis A, Parro Vı Ctor, Moreno-Paz Mercedes, Mellado Rafael P
Abstract excerpt
The Bacillus subtilis 168 csn gene encodes a chitosanase. It was found that transcription of the csn gene was temporally regulated and was not subject to metabolic repression. Chitosanase synthesis was abolished in a csn mutant strain. Csn was overproduced in B. subtilis, partially purified and characterized. The deduced amino acid sequence, K(m), and optimal pH and temperature of the B. subtilis enzyme were...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- DNA Primers
- Genes, Bacterial
- Glycoside Hydrolases
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Biosynthesis
- RNA, Bacterial
- Sequence Homology, Amino Acid
- Streptomyces
- Transcription, Genetic