Article
Diversity of family GH46 chitosanases in Kitasatospora setae KM-6054.
Applied microbiology and biotechnology - 1 Nov 2017
Zitouni Mina, Viens Pascal, Ghinet Mariana G, Brzezinski Ryszard
Abstract excerpt
The genome of Kitasatospora setae KM-6054, a soil actinomycete, has three genes encoding chitosanases belonging to GH46 family. The genes (csn1-3) were cloned in Streptomyces lividans and the corresponding enzymes were purified from the recombinant cultures. The csn2 clone yielded two proteins (Csn2BH and Csn2H) differing by the presence of a carbohydrate-binding domain. Sequence analysis showed that Csn1 and...
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