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Article

Accessory active site residues of Streptomyces sp. N174 chitosanase: variations on a common theme in the lysozyme superfamily.

The FEBS journal - 1 Feb 2009

Lacombe-Harvey Marie-Eve, Fukamizo Tamo, Gagnon Julie, Ghinet Mariana G, Dennhart Nicole, Letzel Thomas, Brzezinski Ryszard

Abstract excerpt

The chitosanase from Streptomyces sp. N174 (CsnN174) is an inverting glycoside hydrolase belonging to family 46. Previous studies identified Asp40 as the general base residue. Mutation of Asp40 into glycine revealed an unexpectedly high residual activity. D40G mutation did not affect the stereochemical mechanism of catalysis or the mode of interaction with substrate. To explain the D40G residual activity,...

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Identifiers

PMID: 19143844
DOI: 10.1111/j.1742-4658.2008.06830.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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