Article
Antisense targeting of engrailed-1 causes abnormal axis formation in mouse embryos.
Teratology - 1 May 1995
Sadler T W, Liu E T, Augustine K A
Abstract excerpt
Antisense oligonucleotide targeting of engrailed-1 (En-1) in early somite mouse embryos resulted in reduced En protein levels and produced abnormalities of the brain, face, and heart and shortening of the embryonic axis (caudal dysgenesis). Defects of the brain and limbs were consistent with abnormalities observed in null mutant mice described by other investigators. Abnormalities of the face and heart may be...
Topics
- Animals
- Base Sequence
- Blotting, Southern
- Brain
- Culture Techniques
- Dose-Response Relationship, Drug
- Embryo, Mammalian
- Embryonic and Fetal Development
- Extremities
- Female
- Genes, Homeobox